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 High Performance Filters for Fluorescence Microscopy

Bandpass Filters

    • Patented Stabilife® Coatings
    • Shift free spectral response @ 0% - 100%RH
    • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles
    • Lowest autofluorescence
    • Extremely sharp transitions from rejection to transmission regions
    • Maximum transmission
    • Excellent set signal-to-noise ratio
    • Optimized optical fabrication for brilliant imaging
    • Superior field life

 
  • Product Description

    HPF™ filter sets for fluorescence microscopy have been manufactured to provide maximum performance using several key design features including exceptionally high passband transmission, maximized out-of-band blocking including extended range blocking on excitation filters, extremely steep slopes from rejection to transmission regions, optical finishing for maximum image rendition including precision grinding and polishing and anti-reflection coatings, and filter bandpass specifications of center wavelength and bandwidth providing maximum image brightness and minimum background.

  • Product Detail
    Newport HPF™ Filters for Microscopy Applications

    Newport's HPF™ series of filters for fluorescence detection is a compilation of product designs of filters that was developed to be used with many of the most popular standard fluorophores and some of the most promising new fluorophores. Since their introduction in the late 1990's, these product designs have served as the starting point for custom designed filters for market leading instruments including DNA Sequencers, Cytometers, MicroPlate Readers, In vivo Imagers, and Thermocyclers. We are now making these OEM proven designs available to the individual researcher through our catalog offering of High Performance Filter sets for Epi-Fluorescence microscopy. HPF™ filters are manufactured using Newport's patented Stabilife® coating technology which provides excellent spectral performance, spectral stability, and physical durability.

    HPF™ filter sets have been manufactured to provide maximum performance using several key design features including exceptionally high passband transmission, maximized out-of-band blocking including extended range blocking on excitation filters, extremely steep slopes from rejection to transmission regions, optical finishing for maximum image rendition including precision grinding and polishing and anti-reflection coatings, and filter bandpass specifications of center wavelength and bandwidth providing maximum image brightness and minimum background.

    Filter Set Selection

    Newport's HPF™ Fluorescence Filter Sets are listed in two tables. The Fluorophore Reference table lists the recommended filter set for each listed fluorophore. The Numerical Reference table identifies all of the fluorophores that can be utilized with each filter set. Spectral performance of each filter set is provided for reference in a series of data curves following the tables.

    Custom Products and Services

    Newport's High Performance Filters for Fluorescence Microscopy are manufactured on a custom basis.  Custom sizes of HPF™ filter sets as well as mounting services are available upon request. Please consult a sales engineer for details.

    Trademark Information

    Alexa Fluor®, BODIPY®, Calcium Crimson™, LysoSensor™,

    MitoTracker®, Oregon Green®, Pacific Blue™, Rhodamine Green™,

    and Texas Red® are trademarks of Molecular Probes, Inc.

    Cy is a trademark of GE Healthcare, Ltd.

    Stabilife®, XM™ and HPF™ are trademarks of Newport Corporation

     

  • Specifications
HPF™ Fluorescence Filters - Typical Specifications
   Excitation Filters  Dichroic Filters  Emission Filters
 Passband Transmittance  ≥90%  ≥90%  ≥90%
 Dichroic Reflectance  N/A  ≥90%  N/A
 Spectral Blocking  ≥OD6  N/A  ≥OD6
 Surface Quality  E/E (60/40) per MIL-F-48616 (typical)  E/E (60/40) per MIL-F-48616 (typical)  E/E (60/40) per MIL-F-48616 (typical)
 Coating Hardness  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Abrasion Resistance  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Adhesion  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Humidity Resistance  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**
 Coating Operating Temperature Range  -100 º C to 300 º C**  -100 º C to 300 º C**  -100 º C to 300 º C**
 Filter Size  25 mm dia.  25.5 mm x 36 mm  25 mm dia.
 Filter Thickness  5 mm  1 mm  5 mm

**The specifications for humidity resistance and coating operating temperature range listed above apply to exposed coatings only. Humidity resistance and operating temperature range of filters manufactured using Stabilife® coatings and assembled using epoxy systems revert to the humidity resistance and operating temperature range of the epoxy system.

HPF™ Fluorescence Filter Sets - Fluorophore Reference
 Fluorophore  Filter Set  Excitation Filter  Dichroic Filter  Emission Filter
 5-FAM  HPF1245  HPX475-44  HPD505  HPM535-50
 5-ROX  HPF1340  HPX560-50  HPD590  HPM640-60
 5-TAMRA  HPF1335  HPX525-45  HPD560  HPM620-75
 6-HEX  HPF1295  HPX520-40  HPD550  HPM590-55
 6-JOE  HPF1230  HPX500-20  HPD515  HPM530LP
 6-TET  HPF1280  HPX500-40  HPD530  HPM575-50
 Alexa Fluor® 350  HPF1205  HPX360-60  HPD400  HPM460-60
 Alexa Fluor® 430  HPF1255  HPX425-40  HPD490  HPM540-40
 Alexa Fluor® 488  HPF1245  HPX475-44  HPD505  HPM535-50
 Alexa Fluor® 532  HPF1275  HPX500-40  HPD530  HPM560-50
 Alexa Fluor® 546  HPF1300  HPX525-45  HPD560  HPM600-60
 Alexa Fluor® 555  HPF1300  HPX525-45  HPD560  HPM600-60
 Alexa Fluor® 568  HPF1345  HPX560-40  HPD595  HPM640-60
 Alexa Fluor® 594  HPF1345  HPX560-40  HPD595  HPM640-60
 Alexa Fluor® 633  HPF1385  HPX610-60  HPD645  HPM685-50
 Alexa Fluor® 647  HPF1400  HPX620-50  HPD660  HPM700-60
 Alexa Fluor® 660  HPF1410  HPX630-60  HPD680  HPM720-60
 Alexa Fluor® 680  HPF1415  HPX660-40  HPD695  HPM725-40
 Alexa Fluor® 700  HPF1420  HPX660-40  HPD695  HPM740-50
 Alexa Fluor® 750  HPF1430  HPX710-50  HPD760  HPM810-80
 AMCA  HPF1205  HPX360-60  HPD400  HPM460-60
 BODIPY® 630/650  HPF1380  HPX605-40  HPD635  HPM670-50
 BODIPY® 650/665  HPF1395  HPX630-40  HPD660  HPM695-50
 BODIPY® FL  HPF1250  HPX480-40  HPD505  HPM535-50
 BODIPY® R6G  HPF1285  HPX510-40  HPD540  HPM575-50
 BODIPY® TMR  HPF1305  HPX530-40  HPD560  HPM600-60
 BODIPY® TR  HPF1355  HPX570-40  HPD610  HPM645-50
 Calcium Crimson™  HPF1365  HPX570-40  HPD595  HPM645-60
 CFP  HPF1220  HPX440-20  HPD455  HPM480-40
 Cy3.5™  HPF1325  HPX565-30  HPD580  HPM620-60
 Cy3™  HPF1310  HPX535-40  HPD565  HPM610-50
 Cy5.5™  HPF1405  HPX645-40  HPD675  HPM710-50
 Cy5™  HPF1390  HPX620-50  HPD655  HPM690-50
 Cy7™  HPF1425  HPX710-50  HPD755  HPM800-70
 DAPI  HPF1205  HPX360-60  HPD400  HPM460-60
 Dil(rs)  HPF1315  HPX535-50  HPD565  HPM620-60
 DiO  HPF1250  HPX480-40  HPD505  HPM535-50
 DsRed  HPF1320  HPX545-30  HPD570  HPM620-60
 Eosin  HPF1270  HPX500-40  HPD525  HPM560-50
 Ethidium Bromide  HPF1350  HPX525-45  HPD565  HPM640-70
 FITC  HPF1250  HPX480-40  HPD505  HPM535-50
 Fluo 3  HPF1250  HPX480-40  HPD505  HPM535-50
 Fluo 4  HPF1250  HPX480-40  HPD505  HPM535-50
 GFP  HPF1240  HPX470-40  HPD495  HPM535-50
 GFP(rs)  HPF1250  HPX480-40  HPD505  HPM535-50
 GFP(wt)  HPF1225  HPX450-50  HPD480  HPM510-50
 Hoechst 33342 & 33258  HPF1200  HPX350-60  HPD400  HPM460-60
 LysoSensor™ Blue (pH 5)  HPF1210  HPX380-40  HPD410  HPM460-60
 MitoTracker® Orange  HPF1335  HPX525-45  HPD560  HPM620-75
 MitoTracker® Red  HPF1340  HPX560-50  HPD590  HPM640-60
 Nile Red  HPF1360  HPX525-45  HPD565  HPM645-60
 Oregon Green™  HPF1260  HPX485-30  HPD515  HPM545-30
 Pacific Blue™  HPF1215  HPX400-30  HPD440  HPM470-30
 PE (B,R)  HPF1290  HPX525-45  HPD560  HPM585-40
 Propidium Iodide  HPF1370  HPX525-45  HPD565  HPM645-75
 Resorufin  HPF1375  HPX560-50  HPD595  HPM645-75
 Rhodamine  HPF1330  HPX570-20  HPD585  HPM620-60
 Rhodamine Green™  HPF1235  HPX500-20  HPD515  HPM535-30
 Texas Red®  HPF1375  HPX560-50  HPD595  HPM645-75
 TRITC  HPF1300  HPX525-45  HPD560  HPM600-60
 YFP  HPF1265  HPX500-20  HPD525  HPM550-40
 YGFP  HPF1235  HPX500-20  HPD515  HPM535-30


HPF™ Fluorescence Filter Sets - Numerical Reference
 Model  Fluorophore  Excitation  Dichroic  Emission
 HPF1200  Hoechst 33342 & 33258  HPX350-60  HPD400  HPM460-60
 HPF1205  Alexa Fluor® 350, AMCA, DAPI  HPX360-60  HPD400  HPM460-60
 HPF1210  LysoSensor™ Blue (pH5)  HPX380-40  HPD410  HPM460-60
 HPF1215  Pacific Blue™  HPX400-30  HPD440  HPM470-30
 HPF1220  CFP  HPX440-20  HPD455  HPM480-40
 HPF1225  GFP (wt)  HPX450-50  HPD480  HPM510-50
 HPF1230  6-JOE  HPX500-20  HPD515  HPM530-LP
 HPF1235  Rhodamine Green™, YGFP  HPX500-20  HPD515  HPM535-30
 HPF1240  GFP  HPX470-40  HPD495  HPM535-50
 HPF1245  5-FAM, Alexa Fluor® 488  HPX475-44  HPD505  HPM535-50
 HPF1250  BODIPY® FL, DiO, FITC, Fluo 3, Fluo 4, GFP (rs)  HPX480-40  HPD505  HPM535-50
 HPF1255  Alexa Fluor® 430  HPX425-40  HPD490  HPM540-40
 HPF1260  Oregon Green™  HPX485-30  HPD515  HPM545-30
 HPF1265  YFP  HPX500-20  HPD525  HPM550-40
 HPF1270  Eosin  HPX500-40  HPD525  HPM560-50
 HPF1275  Alexa Fluor® 532  HPX500-40  HPD530  HPM560-50
 HPF1280  6-TET  HPX500-40  HPD530  HPM575-50
 HPF1285  BODIPY® R6G  HPX510-40  HPD540  HPM575-50
 HPF1290  PE (B,R)  HPX525-45  HPD560  HPM585-40
 HPF1295  6-HEX  HPX520-40  HPD550  HPM590-55
 HPF1300  Alexa Fluor® 546, Alexa Fluor® 555, TRITC  HPX525-45  HPD560  HPM600-60
 HPF1305  BODIPY® TMR  HPX530-40  HPD560  HPM600-60
 HPF1310  Cy3™  HPX535-40  HPD565  HPM610-50
 HPF1315  Dil (rs)  HPX535-50  HPD565  HPM620-60
 HPF1320  DsRed  HPX545-30  HPD570  HPM620-60
 HPF1325  Cy3.5™  HPX565-30  HPD580  HPM620-60
 HPF1330  Rhodamine  HPX570-20  HPD585  HPM620-60
 HPF1335  5-TAMRA, MitoTracker® Orange  HPX525-45  HPD560  HPM620-75
 HPF1340  5-ROX, MitoTracker® Red  HPX560-50  HPD590  HPM640-60
 HPF1345  Alexa Fluor® 568, Alexa Fluor® 594  HPX560-40  HPD595  HPM640-60
 HPF1350  Ethidium Bromide  HPX525-45  HPD565  HPM640-70
 HPF1355  BODIPY® TR  HPX570-40  HPD610  HPM645-50
 HPF1360  Nile Red  HPX525-45  HPD565  HPM645-60
 HPF1365  Calcium Crimson™  HPX570-40  HPD595  HPM645-60
 HPF1370  Propidium Iodide  HPX525-45  HPD565  HPM645-75
 HPF1375  Resorufin, Texas Red®  HPX560-50  HPD595  HPM645-75
 HPF1380  BODIPY® 630/650  HPX605-40  HPD635  HPM670-50
 HPF1385  Alexa Fluor® 633  HPX610-60  HPD645  HPM685-50
 HPF1390  Cy5™  HPX620-50  HPD655  HPM690-50
 HPF1395  BODIPY® 650/665  HPX630-40  HPD660  HPM695-50
 HPF1400  Alexa Fluor® 647  HPX620-50  HPD660  HPM700-60
 HPF1405  Cy5.5™  HPX645-40  HPD675  HPM710-50
 HPF1410  Alexa Fluor® 660  HPX630-60  HPD680  HPM720-60
 HPF1415  Alexa Fluor® 680  HPX660-40  HPD695  HPM725-40
 HPF1420  Alexa Fluor® 700  HPX660-40  HPD695  HPM740-50
 HPF1425  Cy7™  HPX710-50  HPD755  HPM800-70
 HPF1430  Alexa Fluor® 750  HPX710-50  HPD760  HPM810-80

 

 

 
   
   


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